Table 2.TCR-BV sequences identified in brain tissue, CSF cells, and sorted peripheral blood cells (PBL) from Patient A by CDR3 spectratyping
TCR-BV CDR3 sequence
Brain 1996
CSF 2001
CD4+ PBL 2001
CD8+ PBL 2001
CD38+ PBL 2001
BV9 - C A S S - P W E E G - S G N - BJ1.3 + + - - -
BV9 - C A S S - Q I R G V G - E Q Y - BJ2.7 + (+)# - - +
BV16 - C A S - G G T S G K - D T Q - BJ2.3 + (+) - - -
BV17 - C A S S - W A I S D R - Q P Q - BJ1.5
+
(+)
-
-
-

The N-D-N regions are shown in italics, +, completely readable sequence; (+), clonal identity clearly discernible on an oligoclonal background; (+)#, completely readable sequence with clone-specific primer; -, clone not identifiable by CDR3 spectratyping and/or direct sequencing. Note that the TCR sequences observed at different sites and time points were identical both at the nucleotide and amino acid levels