Make Illumina part of your DNA Sign up for PNAS Online eTocs
Link: Info for AuthorsLink: Editorial BoardLink: AboutLink: SubscribeLink: AdvertiseLink: ContactLink: Sitemap Link: PNAS Home
Proceedings of the National Academy of Sciences
Link: Current Issue "" Link: Archives "" Link: Online Submission ""  Link: Advanced Search

Published online on February 27, 2006, 10.1073/pnas.0600035103

This Article
Right arrow Full Text (PDF)
Right arrow Supporting Information
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a colleague
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My File Cabinet
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Biddle, J. F.
Right arrow Articles by Hinrichs, K.-U.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Biddle, J. F.
Right arrow Articles by Hinrichs, K.-U.
Right arrowPubmed/NCBI databases
*Nucleotide
*Compound via MeSH
*Substance via MeSH
Hazardous Substances DB
*CARBON
*CARBON BLACK
GeoRef
Right arrow GeoRef Citation
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg  
What's this?

Microbiology
Environmental Sciences-Physical Sciences
Heterotrophic Archaea dominate sedimentary subsurface ecosystems off Peru

( anaerobic methanotrophy | deep biosphere | FISH-secondary ion MS | intact polar lipids | stable carbon isotopes )

Jennifer F. Biddle a,b, Julius S. Lipp b,c, Mark A. Lever d, Karen G. Lloyd d, Ketil B. Sørensen d, Rika Anderson c,e, Helen F. Fredricks f, Marcus Elvert c, Timothy J. Kelly a,g, Daniel P. Schrag h, Mitchell L. Sogin i, Jean E. Brenchley a,j, Andreas Teske d, Christopher H. House a,g, and Kai-Uwe Hinrichs c,f,k

aPennsylvania State Astrobiology Research Center and Departments of gGeosciences and jBiochemistry and Molecular Biology, Pennsylvania State University, University Park, PA 16802; cOrganic Geochemistry Group, Deutsche Forschungsgemeinschaft Research Center for Ocean Margins and Department of Geosciences, University of Bremen, D-28334 Bremen, Germany; dDepartment of Marine Sciences, University of North Carolina, Chapel Hill, NC 27599; eCarleton College, Northfield, MN 55057; fDepartment of Geology and Geophysics, Woods Hole Oceanographic Institution, Woods Hole, MA 02543; hDepartment of Earth and Planetary Sciences, Harvard University, Cambridge, MA 02138; and iThe Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, Woods Hole, MA 02543

Communicated by John M. Hayes, Woods Hole Oceanographic Institution, Woods Hole, MA, January 5, 2006 (received for review October 22, 2005)

Studies of deeply buried, sedimentary microbial communities and associated biogeochemical processes during Ocean Drilling Program Leg 201 showed elevated prokaryotic cell numbers in sediment layers where methane is consumed anaerobically at the expense of sulfate. Here, we show that extractable archaeal rRNA, selecting only for active community members in these ecosystems, is dominated by sequences of uncultivated Archaea affiliated with the Marine Benthic Group B and the Miscellaneous Crenarchaeotal Group, whereas known methanotrophic Archaea are not detectable. Carbon flow reconstructions based on stable isotopic compositions of whole archaeal cells, intact archaeal membrane lipids, and other sedimentary carbon pools indicate that these Archaea assimilate sedimentary organic compounds other than methane even though methanotrophy accounts for a major fraction of carbon cycled in these ecosystems. Oxidation of methane by members of Marine Benthic Group B and the Miscellaneous Crenarchaeotal Group without assimilation of methane-carbon provides a plausible explanation. Maintenance energies of these subsurface communities appear to be orders of magnitude lower than minimum values known from laboratory observations, and ecosystem-level carbon budgets suggest that community turnover times are on the order of 100-2,000 years. Our study provides clues about the metabolic functionality of two cosmopolitan groups of uncultured Archaea.

Author contributions: A.T., C.H.H., and K.-U.H. designed research; J.F.B., J.S.L., M.A.L., K.G.L., K.B.S., R.A., T.J.K., D.P.S., A.T., C.H.H., and K.-U.H. performed research; M.L.S. contributed new reagents/analytic tools; J.F.B., J.S.L., M.A.L., K.G.L., K.B.S., H.F.F., M.E., J.E.B., A.T., C.H.H., and K.-U.H. analyzed data; and J.F.B., J.S.L., and K.-U.H. wrote the paper.

Conflict of interest statement: No conflicts declared.

bJ.F.B. and J.S.L. contributed equally to this work.

kTo whom correspondence should be addressed at: Organic Geochemistry Group, RCOM and Department of Geosciences, University of Bremen, PO Box 330 440, 28334 Bremen, Germany.

Kai-Uwe Hinrichs, E-mail: khinrichs{at}uni-bremen.de

www.pnas.org/cgi/doi/10.1073/pnas.0600035103
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg    What's this?


This article has been cited by other articles in HighWire Press-hosted journals:


Home page
 Appl. Environ. Microbiol.Home page
S. Schouten, E. C. Hopmans, M. Baas, H. Boumann, S. Standfest, M. Konneke, D. A. Stahl, and J. S. Sinninghe Damste
Intact Membrane Lipids of "Candidatus Nitrosopumilus maritimus," a Cultivated Representative of the Cosmopolitan Mesophilic Group I Crenarchaeota
Appl. Envir. Microbiol., April 15, 2008; 74(8): 2433 - 2440.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
D. Strapoc, F. W. Picardal, C. Turich, I. Schaperdoth, J. L. Macalady, J. S. Lipp, Y.-S. Lin, T. F. Ertefai, F. Schubotz, K.-U. Hinrichs, et al.
Methane-Producing Microbial Community in a Coal Bed of the Illinois Basin
Appl. Envir. Microbiol., April 15, 2008; 74(8): 2424 - 2432.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
D. F. Rodrigues and J. M. Tiedje
Coping with Our Cold Planet
Appl. Envir. Microbiol., March 15, 2008; 74(6): 1677 - 1686.
[Full Text] [PDF]

Home page
 GeologyHome page
M.J. Formolo, J.M. Salacup, S.T. Petsch, A.M. Martini, and K. Nusslein
A new model linking atmospheric methane sources to Pleistocene glaciation via methanogenesis in sedimentary basins
Geology, February 1, 2008; 36(2): 139 - 142.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
T. Losekann, K. Knittel, T. Nadalig, B. Fuchs, H. Niemann, A. Boetius, and R. Amann
Diversity and Abundance of Aerobic and Anaerobic Methane Oxidizers at the Haakon Mosby Mud Volcano, Barents Sea
Appl. Envir. Microbiol., May 15, 2007; 73(10): 3348 - 3362.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
K.-U. Hinrichs, J. M. Hayes, W. Bach, A. J. Spivack, L. R. Hmelo, N. G. Holm, C. G. Johnson, and S. P. Sylva
Biological formation of ethane and propane in the deep marine subsurface
PNAS, October 3, 2006; 103(40): 14684 - 14689.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
K. B. Sorensen and A. Teske
Stratified communities of active archaea in deep marine subsurface sediments.
Appl. Envir. Microbiol., July 1, 2006; 72(7): 4596 - 4603.
[Abstract] [Full Text] [PDF]