Published online on March 10, 2008, 10.1073/pnas.0710834105
OPEN ACCESS ARTICLE
MICROBIOLOGY
Variation in virulence among clades of Escherichia coli O157:H7 associated with disease outbreaks
Shannon D. Manning*,
Alifiya S. Motiwala
,
A. Cody Springman*,
Weihong Qi*,
David W. Lacher*,
Lindsey M. Ouellette*,
Janice M. Mladonicky*,
Patricia Somsel
,
James T. Rudrik,
Stephen E. Dietrich,
Wei Zhang
,
Bala Swaminathan¶,
David Alland, and
Thomas S. Whittam*,||
*Microbial Evolution Laboratory, National Food Safety and Toxicology Center, Michigan State University, East Lansing, MI 48824;
Division of Infectious Diseases, University of Medicine and Dentistry of New Jersey, Newark, NJ 07103;
Bureau of Laboratories, Michigan Department of Community Health, Lansing, MI 48909;
National Center for Food Safety and Technology, Illinois Institute of Technology, Summit, IL 60501; and
¶Foodborne and Diarrheal Diseases Branch, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333
Edited by Masatoshi Nei, Pennsylvania State University, University Park, PA, and approved January 25, 2008 (received for review November 16, 2007)
Abstract
Escherichia coli O157:H7, a toxin-producing food and waterborne bacterial pathogen, has been linked to large outbreaks of gastrointestinal illness for more than two decades. E. coli O157 causes a wide range of clinical illness that varies by outbreak, although factors that contribute to variation in disease severity are poorly understood. Several recent outbreaks involving O157 contamination of fresh produce (e.g., spinach) were associated with more severe disease, as defined by higher hemolytic uremic syndrome and hospitalization frequencies, suggesting that increased virulence has evolved. To test this hypothesis, we developed a system that detects SNPs in 96 loci and applied it to >500 E. coli O157 clinical strains. Phylogenetic analyses identified 39 SNP genotypes that differ at 20% of SNP loci and are separated into nine distinct clades. Differences were observed between clades in the frequency and distribution of Shiga toxin genes and in the type of clinical disease reported. Patients with hemolytic uremic syndrome were significantly more likely to be infected with clade 8 strains, which have increased in frequency over the past 5 years. Genome sequencing of a spinach outbreak strain, a member of clade 8, also revealed substantial genomic differences. These findings suggest that an emergent subpopulation of the clade 8 lineage has acquired critical factors that contribute to more severe disease. The ability to detect and rapidly genotype O157 strains belonging to such lineages is important and will have a significant impact on both disease diagnosis and treatment guidelines.
pathogens | polymorphisms | population genetics
Footnotes
Author contributions: D.A. and T.S.W. designed research; S.D.M., A.S.M., A.C.S., L.M.O., and J.M.M. performed research; S.D.M., A.S.M., W.Q., D.W.L., P.S., J.T.R., S.E.D., W.Z., and B.S. analyzed data; and S.D.M. and T.S.W. wrote the paper.
The authors declare no conflict of interest.
This article is a PNAS Direct Submission.
||To whom correspondence should be addressed. E-mail: whittam{at}msu.edu
© 2008 by The National Academy of Sciences of the USA
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg What's this?
This article has been cited by other articles in HighWire Press-hosted journals:
|
|
|
|
 
Understanding E. coli O157:H7
Journal Watch Infectious Diseases,
April 16, 2008;
2008(416):
2 - 2.
[Full Text]
|
|
|
|
|
|
|
J. B. Kaper and M. A. Karmali
The continuing evolution of a bacterial pathogen
PNAS,
March 25, 2008;
105(12):
4535 - 4536.
[Full Text]
[PDF]
|
|
|
