Complete, 12-subunit RNA polymerase II at 4.1-Å resolution: Implications for the initiation of transcription
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Edited by E. Peter Geiduschek, University of California at San Diego, La Jolla, CA, and approved April 3, 2003 (received for review January 30, 2003)
Abstract
The x-ray structure of complete RNA polymerase II from Saccharomyces cerevisiae has been determined, including a heterodimer of subunits Rpb4 and Rpb7 not present in previous “core” polymerase II structures. The heterodimer maintains the polymerase in the conformation of a transcribing complex, may bind RNA as it emerges from the enzyme, and is in a position to interact with general transcription factors and the Mediator of transcriptional regulation.
Footnotes
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↵ * To whom correspondence should be addressed. E-mail: kornberg{at}stanford.edu.
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This paper was submitted directly (Track II) to the PNAS office.
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Abbreviations: pol II, polymerase II; EM, electron microscopy; CTD, C-terminal repeat domain; OB, oligonucleotide binding; RNP, ribonucleoprotein.
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Data deposition: The atomic coordinates and structure factors have been deposited in the Protein Data Bank, www.rcsb.org (PDB ID code 1NIK).
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See commentary on page 6893.
- Copyright © 2003, The National Academy of Sciences
