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Published online on May 23, 2003, 10.1073/pnas.1232225100
PNAS | June 24, 2003 | vol. 100 | no. 13 | 7865-7870


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From The Cover
Microbiology
Division site selection in Escherichia coli involves dynamic redistribution of Min proteins within coiled structures that extend between the two cell poles

Yu-Ling Shih, Trung Le, and Lawrence Rothfield *

Department of Microbiology, University of Connecticut Health Center, Farmington, CT 06032

Communicated by M. J. Osborn, University of Connecticut Health Center, Farmington, CT, April 15, 2003 (received for review March 20, 2003)

The MinCDE proteins of Escherichia coli are required for proper placement of the division septum at midcell. The site selection process requires the rapid oscillatory redistribution of the proteins from pole to pole. We report that the three Min proteins are organized into extended membrane-associated coiled structures that wind around the cell between the two poles. The pole-to-pole oscillation of the proteins reflects oscillatory changes in their distribution within the coiled structure. We also report that the E. coli MreB protein, which is required for maintaining the rod shape of the cell, also forms extended coiled structures, which are similar to the MreB structures that have previously been reported in Bacillus subtilis. The MreB and MinCDE coiled arrays do not appear identical. The results suggest that at least two functionally distinct cytoskeletal-like elements are present in E. coli and that structures of this type can undergo dynamic changes that play important roles in division site placement and possibly other aspects of the life of the cell.

Abbreviations: YFP, yellow fluorescent protein; CFP, cyan fluorescent protein.

See commentary on page 7423.

* To whom correspondence should be addressed. E-mail: lroth{at}neuron.uchc.edu.


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Bacterial cell division spirals into control
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