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GENETICS
Adaptive mutation: General mutagenesis is not a programmed response to stress but results from rare coamplification of dinB with lac

E. Susan Slechta ^*, Kim L. Bunny ^*, Elisabeth Kugelberg  , Eric Kofoid ^*, Dan I. Andersson  , and John R. Roth ^* 

^*Microbiology Section, Division of Biological Sciences, University of California, Davis, CA 95616; Department of Bacteriology, Swedish Institute for Infectious Disease Control, S-171 82 Solna, Sweden; and Karolinska Institute of Microbiology, Tumour Biology Center, S-171 77 Solna, Sweden

Contributed by John R. Roth, August 25, 2003

In a particular genetic system, selection stimulates reversion of a lac mutation and causes genome-wide mutagenesis (adaptive mutation). Selection allows rare plated cells with a duplication of the leaky lac allele to initiate clones within which further lac amplification improves growth rate. Growth and amplification add mutational targets to each clone and thereby increase the likelihood of reversion. We suggest that general mutagenesis occurs only in clones whose lac amplification includes the nearby dinB⁺ gene (for error-prone DNA polymerase IV). Thus mutagenesis is not a programmed response to stress but a side effect of amplification in a few clones; it is not central to the effect of selection on reversion.

To whom correspondence should be addressed. E-mail: jrroth{at}ucdavis.edu.

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