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Published online on July 13, 2004, 10.1073/pnas.0403849101
PNAS | July 27, 2004 | vol. 101 | no. 30 | 10979-10983


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From the Cover
BIOPHYSICS
The dynamics of genomic-length DNA molecules in 100-nm channels

Jonas O. Tegenfeldt *, Christelle Prinz *, Han Cao {dagger}, Steven Chou {dagger}, Walter W. Reisner {ddagger}, Robert Riehn {ddagger}, Yan Mei Wang {ddagger}, Edward C. Cox §, James C. Sturm {dagger}, Pascal Silberzan ¶, and Robert H. Austin {ddagger}, ||

*Department of Physics, Lund University, SE-221 00 Lund, Sweden; Departments of {dagger}Electrical Engineering, {ddagger}Physics, and §Molecular Biology, Princeton University, Princeton, NJ 08544; and Institut Curie, Centre National de la Recherche Scientifique, 75231 Paris, France

Contributed by Robert H. Austin, June 2, 2004

We show that genomic-length DNA molecules imaged in nanochannels have an extension along the channel that scales linearly with the contour length of the polymer, in agreement with the scaling arguments developed by de Gennes for self-avoiding confined polymers. This fundamental relationship allows us to measure directly the contour length of single DNA molecules confined in the channels, and the statistical analysis of the dynamics of the polymer in the nanochannel allows us to compute the SD of the mean of the extension. This statistical analysis allows us to measure the extension of {lambda} DNA multimers with a 130-nm SD in 1 min.

|| To whom correspondence should be addressed. E-mail: austin{at}princeton.edu.

© 2004 by The National Academy of Sciences of the USA


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