Single-nucleotide polymorphism detection with “wire-like” DNA probes that display quasi “on–off” digital action
- †Precursory Research for Embryonic Science and Technology, Japan Science and Technology Agency, and ‡Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Sugitani 2630, Toyama 930-0194, Japan
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Edited by Joshua Jortner, Tel Aviv University, Tel Aviv, Israel, and approved June 24, 2005 (received for review March 14, 2005)
Abstract
Establishing a reliable genotyping protocol is a critical matter in postsequence genetics. In this article, we describe a highly sensitive electrochemical detection of complementary DNAs (up to 43-mer) based on hole transport with molecular-scale, “wire-like” DNA probes. The presence of a single-base mismatch in the DNA duplexes caused a dramatic decrease in the electrochemical response. We applied this method to detect all of the possible transition and transversion SNPs and achieved “on–off”-type discrimination of fully complementary DNAs from their SNPs. Furthermore, naturally occurring polymorphisms, “hot spots” from the p53 gene, could clearly be distinguished from wild type by using our methodology.
Footnotes
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↵ § To whom correspondence should be addressed. E-mail: inouye{at}ms.toyama-mpu.ac.jp.
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Author contributions: M.I. designed research; M.I., R.I., M.T., T.T., and J.C. performed research; M.I., R.I., M.T., and T.T. contributed new reagents/analytic tools; M.I., R.I., M.T., and J.C. analyzed data; and M.I. and J.C. wrote the paper.
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This paper was submitted directly (Track II) to the PNAS office.
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Abbreviations: Fc, ferrocene unit with acetylene linker; Fc2, ferrocene unit with ethane linker; SWV, square wave voltammetry.
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Freely available online through the PNAS open access option.
- Copyright © 2005, The National Academy of Sciences
