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Published online on December 12, 2005, 10.1073/pnas.0506415102
PNAS | December 20, 2005 | vol. 102 | no. 51 | 18350-18355


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BIOCHEMISTRY
Human La is found at RNA polymerase III-transcribed genes in vivo

Jennifer A. Fairley *, Theodoros Kantidakis *, Niall S. Kenneth *, Robert V. Intine {dagger}, Richard J. Maraia {dagger}, and Robert J. White *, {ddagger}

*Institute of Biomedical and Life Sciences, Division of Biochemistry and Molecular Biology, University of Glasgow, Glasgow G12 8QQ, United Kingdom; and {dagger}Laboratory of Molecular Growth Regulation, National Institute of Child Health and Development, Bethesda, MD 20892

Edited by Mark T. Groudine, Fred Hutchinson Cancer Research Center, Seattle, WA, and approved October 20, 2005 (received for review July 27, 2005)

The human La autoantigen can bind to nascent RNA transcripts and has also been postulated to act as an RNA polymerase III (pol III) transcription initiation and termination factor. Here, we show by chromatin immunoprecipitation (ChIP) that La is associated with pol III-transcribed genes in vivo. In contrast, the Ro autoantigen, which can also bind pol III transcripts, is not found at these genes. The putative pol III transcription factors NF1 and TFIIA are also not detected at class III genes. Binding of La remains when transcription is repressed at mitosis and does not correlate with the presence of polymerase at the gene. However, gene occupancy depends on the phosphorylation status of La, with the less prevalent, unphosphorylated form being found selectively on pol III templates.

La | NF1 | TFIIA | chromatin immunoprecipitation | RNA polymerase III transcription

Author contributions: J.A.F. and R.J.W. designed research; J.A.F., T.K., N.S.K., and R.V.I. performed research; R.J.M. contributed new reagents/analytic tools; J.A.F. and R.J.W. analyzed data; and J.A.F. and R.J.W. wrote the paper.

Conflict of interest statement: No conflicts declared.

This paper was submitted directly (Track II) to the PNAS office.

Abbreviations: snRNA, small nuclear RNA; pol III, RNA polymerase III; pLa, phosphorylated La; npLa, nonphosphorylated La; ChIP, chromatin immunoprecipitation; ARPP, acidic ribosomal phosphoprotein; CSF1, colony-stimulating factor 1.

{ddagger} To whom correspondence should be addressed. E-mail: rwhite{at}udcf.gla.ac.uk.

© 2005 by The National Academy of Sciences of the USA


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