Ca²⁺ blinks: Rapid nanoscopic store calcium signaling

Abstract

Luminal Ca²⁺ in the endoplasmic and sarcoplasmic reticulum (ER/SR) plays an important role in regulating vital biological processes, including store-operated capacitative Ca²⁺ entry, Ca²⁺-induced Ca²⁺ release, and ER/SR stress-mediated cell death. We report rapid and substantial decreases in luminal [Ca²⁺], called “Ca²⁺ blinks,” within nanometer-sized stores (the junctional cisternae of the SR) during elementary Ca²⁺ release events in heart cells. Blinks mirror small local increases in cytoplasmic Ca²⁺,orCa²⁺ sparks, but changes of [Ca²⁺] in the connected free SR network were below detection. Store microanatomy suggests that diffusional strictures may account for this paradox. Surprisingly, the nadir of the store depletion trails the peak of the spark by about 10 ms, and the refilling of local store occurs with a rate constant of 35 s^-1, which is ≈6-fold faster than the recovery of local Ca²⁺ release after a spark. These data suggest that both local store depletion and some time-dependent inhibitory mechanism contribute to spark termination and refractoriness. Visualization of local store Ca²⁺ signaling thus broadens our understanding of cardiac store Ca²⁺ regulation and function and opens the possibility for local regulation of diverse store-dependent functions.

• calcium-induced calcium release
• calcium spark
• cardiac myocytes
• endoplasmic reticulum
• sarcoplasmic reticulum