Submit Papers Directly to PNAS via Track II Sign up for PNAS Online eTocs
Link: Info for AuthorsLink: Editorial BoardLink: AboutLink: SubscribeLink: AdvertiseLink: ContactLink: Sitemap Link: PNAS Home
Proceedings of the National Academy of Sciences
Link: Current Issue "" Link: Archives "" Link: Online Submission ""  Link: Advanced Search

Published online on May 8, 2006, 10.1073/pnas.0602646103
PNAS | May 16, 2006 | vol. 103 | no. 20 | 7801-7806


This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supporting Information
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a colleague
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My File Cabinet
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via ISI Web of Science (8)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zeng, F.
Right arrow Articles by Huang, S.-z.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zeng, F.
Right arrow Articles by Huang, S.-z.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg  
What's this?

 Previous Article  | Table of Contents |  Next Article 

BIOLOGICAL SCIENCES / MEDICAL SCIENCES
Multiorgan engraftment and differentiation of human cord blood CD34+Lin– cells in goats assessed by gene expression profiling

Fanyi Zeng*, Mei-jue Chen*, Don A. Baldwin{dagger}, Zhi-juan Gong*, Jing-bin Yan*, Hui Qian*, Juan Wang*, Xiaoyan Jiang*,{ddagger},§, Zhao-rui Ren*, Deming Sun*, and Shu-zhen Huang*,¶

*Shanghai Institute of Medical Genetics, Shanghai Children's Hospital, Shanghai Jiao Tong University, 24/1400 West Beijing Road, Shanghai 200040, People's Republic of China; {dagger}Penn Microarray Facility, University of Pennsylvania, Philadelphia, PA 19104; and {ddagger}Terry Fox Laboratory, British Columbia Cancer Agency and §Department of Medical Genetics, University of British Columbia, Vancouver, BC, Canada V5Z 1L3

Communicated by Jiazhen Tan, Fudan University, Shanghai, People's Republic of China, April 3, 2006 (received for review December 8, 2005)

To investigate multitissue engraftment of human primitive hematopoietic cells and their differentiation in goats, human CD34+Lin– cord blood cells transduced with a GFP vector were transplanted into fetal goats at 45–55 days of gestation. GFP+ cells were detected in hematopoietic and nonhematopoietic organs including blood, bone marrow, spleen, liver, kidney, muscle, lung, and heart of the recipient goats (1.2–36% of all cells examined). We identified human beta2 microglobulin-positive cells in multiple tissues. GFP+ cells sorted from the perfused liver of a transplant goat showed human insulin-like growth factor 1 gene sequences, indicating that the engrafted GFP+ cells were of human origin. A substantial fraction of cells engrafted in goat livers expressed the human hepatocyte-specific antigen, proliferating cell nuclear antigen, albumin, hepatocyte nuclear factor, and GFP. DNA content analysis showed no evidence for cellular fusion. Long-term engraftment of GFP+ cells could be detected in the blood of goats for up to 2 yr. Microarray analysis indicated that human genes from a variety of functional categories were expressed in chimeric livers and blood. The human/goat xenotransplant model provides a unique system to study the kinetics of hematopoietic stem cell engraftment, gene expression, and possible stem cell plasticity under noninjured conditions.

hematopoietic stem cell | transplantation | plasticity | microarray

Author contributions: F.Z., D.S., and S.-z.H. designed research; F.Z., M.-j.C., D.A.B., Z.-j.G., J.-b.Y., H.Q., J.W., X.J., and S.-z.H. performed research; F.Z., D.A.B., and S.-z.H. contributed new reagents/analytic tools; F.Z., M.-j.C., D.A.B., and S.-z.H. analyzed data; and F.Z., D.A.B., X.J., Z.-r.R., D.S., and S.-z.H. wrote the paper.

Conflict of interest statement: No conflicts declared.

Data deposition: The data described in this paper have been deposited in the Gene Expression Omnibus (GEO) database, www.ncbi.nlm.nih.gov/geo (accession no. GDS1023).

To whom correspondence should be addressed. E-mail: szhuang{at}simg.org or ytzeng{at}stn.sh.cn

© 2006 by The National Academy of Sciences of the USA


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg    What's this?


This article has been cited by other articles in HighWire Press-hosted journals:


Home page
 Hum Mol GenetHome page
S.-Y. Xie, Z.-R. Ren, J.-Z. Zhang, X.-B. Guo, Q.-X. Wang, S. Wang, D. Lin, X.-L. Gong, W. Li, S.-Z. Huang, et al.
Restoration of the balanced {alpha}/{beta}-globin gene expression in {beta}654-thalassemia mice using combined RNAi and antisense RNA approach
Hum. Mol. Genet., November 1, 2007; 16(21): 2616 - 2625.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
G. C. Schatteman, M. Dunnwald, and C. Jiao
Biology of bone marrow-derived endothelial cell precursors
Am J Physiol Heart Circ Physiol, January 1, 2007; 292(1): H1 - H18.
[Abstract] [Full Text] [PDF]