Chromatin remodeling by nucleosome disassembly in vitro
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Contributed by Roger D. Kornberg, December 21, 2005
Abstract
The RSC chromatin-remodeling complex completely disassembles a nucleosome in the presence of the histone chaperone Nap1 and ATP. Disassembly occurs in a stepwise manner, with the removal of H2A/H2B dimers, followed by the rest of the histones and the release of naked DNA. RSC and related chromatin-remodeling complexes may be responsible for the removal of promoter nucleosomes during transcriptional activation in vivo.
Footnotes
- *To whom correspondence should be addressed. E-mail: kornberg{at}stanford.edu
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Author contributions: Y.L. and R.D.K. designed research; Y.L. and B.M.-D. performed research; Y.L. analyzed data; and Y.L. and R.D.K. wrote the paper.
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Conflict of interest statement: No conflicts declared.
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Freely available online through the PNAS open access option.
- © 2006 by The National Academy of Sciences of the USA
