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BIOLOGICAL SCIENCES / PLANT BIOLOGY
Omics-based identification of Arabidopsis Myb transcription factors regulating aliphatic glucosinolate biosynthesis
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*RIKEN Plant Science Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan; Kazusa DNA Research Institute, 2-6-7 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan; Graduate School of Pharmaceutical Sciences, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba, Chiba 263-8522, Japan; Core Research for Evolutional Science and Technology, Japan Science and Technology Agency, Saitama 332-0012, Japan; and ¶Central Laboratories for Frontier Technology, Kirin Brewery Company, Ltd., 1-13-5 Fukuura, Kanazawa-ku, Yokohama, Kanagawa 236-0004, Japan
Edited by Marc C. E. Van Montagu, Ghent University, Ghent, Belgium, and approved February 13, 2007 (received for review December 28, 2006)
Understanding plant metabolism as an integrated system is essential for metabolic engineering aimed at the effective production of compounds useful to human life and the global environment. The "omics" approach integrates transcriptome and metabolome data into a single data set and can lead to the identification of unknown genes and their regulatory networks involved in metabolic pathways of interest. One of the intriguing, although poorly described metabolic pathways in plants is the biosynthesis of glucosinolates (GSLs), a group of bioactive secondary products derived from amino acids that are found in the family Brassicaceae. Here we report the discovery of two R2R3-Myb transcription factors that positively control the biosynthesis of GSLs in Arabidopsis thaliana by an integrated omics approach. Combined transcriptome coexpression analysis of publicly available, condition-independent data and the condition-specific (i.e., sulfur-deficiency) data identified Myb28 and Myb29 as candidate transcription factor genes specifically involved in the regulation of aliphatic GSL production. Analysis of a knockout mutant and ectopic expression of the gene demonstrated that Myb28 is a positive regulator for basal-level production of aliphatic GSLs. Myb29 presumably plays an accessory function for methyl jasmonate-mediated induction of a set of aliphatic GSL biosynthetic genes. Overexpression of Myb28 in Arabidopsis-cultured suspension cells, which do not normally synthesize GSLs, resulted in the production of large amounts of GSLs, suggesting the possibility of efficient industrial production of GSLs by manipulation of these transcription factors. A working model for regulation of GSL production involving these genes, renamed Production of Methionine-Derived Glucosinolate (PMG) 1 and 2, are postulated.
coexpression | functional genomics | transcriptomics
Author contributions: M.Y.H., K. Sugiyama, and Y.S. contributed equally to this work; M.Y.H. and K. Saito designed research; K. Sugiyama, Y.S., T.T., A.S., R.A., and H.G. performed research; N.S., H.S., K.A., and D.S. contributed new reagents/analytic tools; M.Y.H., K. Sugiyama, Y.S., T.T., T.O., and O.I.N. analyzed data; and M.Y.H. and K. Saito wrote the paper.
The authors declare no conflict of interest.
This article is a PNAS Direct Submission.
Data deposition: Microarray data have been deposited in ArrayExpress database (accession nos. E-ATMX-6, E-ATMX-7, and E-ATMX-8).
This article contains supporting information online at www.pnas.org/cgi/content/full/0611629104/DC1.
||To whom correspondence should be addressed. E-mail: ksaito{at}psc.riken.jp
© 2007 by The National Academy of Sciences of the USA
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