Nucleotide sequence and predicted functions of the entire Sinorhizobium meliloti pSymA megaplasmid
- Melanie J. Barnetta,
- Robert F. Fishera,
- Ted Jonesb,
- Caridad Kompb,
- A. Pia Abolab,
- Frédérique Barloy-Hublerc,
- Leah Bowserb,
- Delphine Capelac,d,e,
- Francis Galibertc,
- Jérôme Gouzyd,
- Mani Gurjalb,
- Andrea Honga,
- Lucas Huizarb,
- Richard W. Hymanb,
- Daniel Kahnd,
- Michael L. Kahnf,
- Sue Kalmanb,g,
- David H. Keatinga,h,
- Curtis Palmb,
- Melicent C. Pecka,
- Raymond Surzyckib,i,
- Derek H. Wellsa,
- Kuo-Chen Yeha,h,j,
- Ronald W. Davisb,
- Nancy A. Federspielb,k, and
- Sharon R. Longa,h,l
- aDepartment of Biological Sciences, and hHoward Hughes Medical Institute, Stanford University, Stanford, CA 94305; bStanford Center for DNA Sequencing and Technology, 855 California Avenue, Palo Alto, CA 94304; cLaboratoire de Génétique et Développement, Faculté de Médecine, 2 Avenue du Pr. Léon Bernard, F-35043 Rennes Cedex, France; dLaboratoire de Biologie Moléculaire de Relations Plantes–Microorganisms, Unité Mixte de Recherche, 215 Institut National de la Recherche Agronomique–Centre National de la Recherche Scientifique, F-31326 Castanet Tolosan, France; and fInstitute of Biological Chemistry, Washington State University, Pullman, WA 99164
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Contributed by Sharon R. Long
Abstract
The symbiotic nitrogen-fixing soil bacterium Sinorhizobium meliloti contains three replicons: pSymA, pSymB, and the chromosome. We report here the complete 1,354,226-nt sequence of pSymA. In addition to a large fraction of the genes known to be specifically involved in symbiosis, pSymA contains genes likely to be involved in nitrogen and carbon metabolism, transport, stress, and resistance responses, and other functions that give S. meliloti an advantage in its specialized niche.
Footnotes
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↵ e Present address: Institut Curie, 26 Rue d'Ulm, 75005 Paris, France.
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↵ g Present address: Incyte Genomics, 3160 Porter Drive, Palo Alto, CA 94304.
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↵ i Present address: Département de Biologie Moléculaire Sciences 2, Université de Genève, Geneva 1211, Switzerland.
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↵ j Present address: Institute of BioAgricultural Sciences, Academia Sinica, Nankang, Taipei 11529, Taiwan.
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↵ k Present address: Exelixis, Inc., 170 Harbor Way, P.O. Box 511, South San Francisco, CA 94083-0511.
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↵ l To whom reprint requests should be addressed. E-mail: srl{at}leland.stanford.edu.
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Data deposition: The sequence reported in this paper has been deposited in the GenBank database (accession no. AE006469).
- Abbreviations:
- GABA,
- 4-aminobutyrate;
- FDH,
- formate dehydrogenase;
- Mb,
- megabase
- Copyright © 2001, The National Academy of Sciences
