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STRAND-SPECIFIC TRANSFER OF DONOR DNA DURING CONJUGATION IN E. coli*
Abstract
After conjugation of E. coli, only the preformed donor DNA strand with a leading 5′ end can be recovered from the recipient. DNA that was transferred to recipients from radioactive-labeled donor strains lysogenic for λ bacteriophage was recovered as radioactive material in mature phage particles. The radioactivity was recovered in strand l when gene transfer was in the order gal-λ-bio, and in strand r when the order of transfer was bio-λ-gal. The assignment of the 5′ end as the origin of the transferred strand follows directly from the known orientation of prophage λ in the chromosome.
Footnotes
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↵ † Present address: Radiology Laboratories, Yale University School of Medicine, New Haven, Conn. 06510.
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↵ * This work was supported by the following grants: NSF GB 6082 and NIH A108186. G.I. was supported by USPHS grant 1-F2-GM-13, 855-01. W.D.R., on leave of absence from the Department of Radiology, Yale University School of Medicine, was supported by USPHS grant CA06519 to that department.
