ISOLATION OF ADENYL CYCLASE FROM Escherichia coli*

  1. Mariano Tao† and
  2. Fritz Lipmann
  1. THE ROCKEFELLER UNIVERSITY

Abstract

We have found a soluble cyclase, using for assay radioactively marked ATP as precursor. The reaction product was isolated by thin-layer chromatography and identified by specific degradation. After homogenization, part of the activity remained in the particulate fraction but could be easily extracted. The cyclase was concentrated 100-fold by conventional methods. The enzyme has a Mg++ requirement and is inhibited by fluoride and inorganic pyrophosphate.

Footnotes

  • ↵ † Aided by a grant, no. PF-506, from the American Cancer Society.

  • ↵ * This work was supported, in part, by a grant from the USPHS, no. GM-13972.

« Previous | Next Article »Table of Contents

This Article

  1. PNAS 1969-05 vol. 63 no. 1 86-92
  1. » Abstract
  2. Full Text (PDF)
About Our New Site Design >>
Link: Advanced Search

This Week's Issue

  1. July 1, 2008, 105 (26)
  1. Current Issue
  1. Alert me to new issues of PNAS

Most