THE PROCESS OF INFECTION WITH BACTERIOPHAGE ϕX174, XXX. REPLICATION OF DOUBLE-STRANDED ϕX DNA*

  1. Rolf Knippers†,
  2. J. Millar Whalley, and
  3. Robert L. Sinsheimer
  1. DIVISION OF BIOLOGY, CALIFORNIA INSTITUTE OF TECHNOLOGY, PASADENA

Abstract

Intermediates involved in the replication of double-stranded ϕX174 RF DNA have been identified and partially characterized. Analysis of pulselabeled RF DNA suggests that the synthesis of progeny RF molecules involves, in part, the addition of nucleotides to linear complementary strands on a circular parental strand as template, so as to produce intermediate DNA strands of greater than viral length. Electron microscopy reveals DNA rings with “tails” and “double rings,” which could be the intermediate structures. A model is postulated for the replication process.

Footnotes

  • ↵ † Present address: Max-Planck-Institut für Med. Forschung, Heidelberg, West Germany.

  • ↵ * This research was supported in part by grant GM 13554 from the U.S. Public Health Service.

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  1. PNAS 1969-09 vol. 64 no. 1 275-282
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