ISOLATION AND PARTIAL CHARACTERIZATION OF A MAJOR INOSITOL-CONTAINING LIPID IN BAKER'S YEAST, MANNOSYL-DIINOSITOL, DIPHOSPHORYL-CERAMIDE*

  1. S. Steiner†,
  2. Sharron Smith‡,
  3. C. J. Waechter§, and
  4. Robert L. Lester
  1. DEPARTMENT OF BIOCHEMISTRY, UNIVERSITY OF KENTUCKY, LEXINGTON

Abstract

A polar lipid containing about 20 per cent of the lipid soluble inositol has been purified from cells of Saccharomyces cerevisiae. This compound labeled with 3H-inositol, 32Pi, or 14C glucose was isolated from aqueous pyridine extracts by means of paper chromatography. Unlabeled compound was obtained by a differential solubility scheme. Analytical and degradation studies suggest that this compound can be formulated as a mannosyl-diinositol, diphosphoryl-ceramide. It can be converted by treatment with base or acid into a mannosylmonoinositol phosphoryl-ceramide, a composition which relates it to the basic structure of the phytoglycolipids.

Footnotes

  • ↵ † Postdoctoral fellow U.S. Public Health Service 1FO2GM3B109-02A1.

  • ↵ ‡ Recipient of a National Defense Graduate Fellowship.

  • ↵ § Predoctoral trainee of the U.S. Public Health Service under training grant GM01026.

  • ↵ * This investigation was supported by U.S. Public Health Service grant NB08323.

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  1. PNAS 1969-11 vol. 64 no. 3 1042-1048
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