THE ELECTION TRANSPORT SYSTEM IN NITROGEN FIXATION BY Azotobacter, I. AZOTOFLAVIN AS AN ELECTRON CARRIER*

  1. J. R. Benemann,
  2. D. C. Yoch,
  3. R. C. Valentine, and
  4. D. I. Arnon
  1. DEPARTMENT OF BIOCHEMISTRY, UNIVERSITY OF CALIFORNIA, BERKELEY
  2. DEPARTMENT OF CELL PHYSIOLOGY, UNIVERSITY OF CALIFORNIA, BERKELEY

Abstract

A flavoprotein, named azotoflavin, was isolated from an extract of Azotobacter vinelandii cells, which linked the reducing power generated by illuminated spinach chloroplasts to the Azotobacter nitrogen-fixing enzyme complex. The photoreduction of the yellow azotoflavin by chloroplasts produced a stable, free-radical semiquinone, blue in color, with properties similar to those described by other investigators for an Azotobacter flavoprotein of unknown biological function.

Footnotes

  • ↵ * This investigation was aided by grants to D. I. A. and R. C. V. from the National Science Foundation.

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  1. PNAS 1969-11 vol. 64 no. 3 1079-1086
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