In Vitro Excision-Repair of Ultraviolet-Irradiated Transforming DNA from Bacillus subtilis

  1. H. L. Heijneker†,*,
  2. H. Pannekoek†,
  3. R. A. Oosterbaan†,
  4. P. H. Pouwels†,
  5. S. Bron‡,
  6. F. Arwert‡, and
  7. G. Venema‡
  1. †Medical Biological Laboratory TNO, Rijswijk (ZH), The Netherlands
  2. ‡Institute of Genetics, State University of Groningen, Haren, P.O. Box 14, The Netherlands

Abstract

The biological activity of UV-inactivated Bacillus subtilis DNA is partly restored after incubation with a UV-specific endonuclease from Micrococcus lutens in conjunction with DNA polymerase and DNA ligase, both isolated from Escherichia coli. The restored activity is not further increased by photoreactivation. Pyrimidine dimers are specifically liberated when irradiated DNA is exposed to the three enzymes. None of these effects is observed when pancreatic DNase is used instead of UV-specific endonuclease.

Footnotes

  • ↵ * Laboratory for Molecular Genetics, Leiden State University. Temporary address: P.O. Box 45, Rijswijk 2100, The Netherlands.

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  1. PNAS 1971-12 vol. 68 no. 12 2967-2971
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