The Leader Sequence from the 5′-Terminus to the A-Protein Initiation Codon in MS2-Virus RNA

  1. R. De Wachter,
  2. A. Vandenberghe,
  3. J. Merregaert,
  4. R. Contreras, and
  5. W. Fiers
  1. Laboratory of Physiological Chemistry, University of Ghent, Belgium
  2. Laboratory of Molecular Biology, University of Ghent, Belgium

Abstract

RNA fragments of different chain length, each containing the 5′-terminal guanosine tetraphosphate (pppGp) of bacteriophage-MS2 RNA, have been isolated from partial ribonuclease digests of the viral RNA. The longest fragment overlaps with the ribosomalbinding site of the A-protein cistron. The base sequence has been established for the major part. The results directly confirm that the A-protein cistron is closest to the 5′-terminus. Its initiating (AUG) codon starts at position 130, being preceded by an untranslated sequence of 129 nucleotides.

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  1. PNAS 1971-03 vol. 68 no. 3 585-589
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