Mucopolysaccharide and Protein—Polysaccharide of a Transplantable Rat Chondrosarcoma

  1. Haing Ug Choi,
  2. Karl Meyer, and
  3. Richard Swarm
  1. Department of Chemistry, Belfer Graduate School of Science, Yeshiva University, New York 10033
  2. Research Division, Hoffman La Roche, Inc., Nutley, New Jersey

Abstract

Two mucopolysaccharides, chondroitin 4-sulfate (97.8%) and hyaluronic acid (1.2%), were isolated after exhaustive proteolysis of a transplantable chondrosarcoma of the rat. The chondroitin 4-sulfate was fractionated into three fractions of varying degrees of sulfation and chain length. Keratan sulfate and chondroitin 6-sulfate were absent. Extraction of the fresh tumor gave two protein—polysaccharides of similar carbohydrate composition, one soluble in 0.5 M NaCl, the other insoluble. The latter was solubilized in 4 M guanidine·HCl. A dialyzable fraction from the 4 M guanidine solution may be responsible for the insolubility. Both protein—polysaccharides were antigenic and cross-reacted with similar fractions of bovine and human cartilage.

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  1. PNAS 1971-05 vol. 68 no. 5 877-879
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