Effect of Colicin E3 upon the 30S Ribosomal Subunit of Escherichia coli

  1. B. W. Senior and
  2. I. B. Holland
  1. Department of Genetics, University of Leicester, England

Abstract

The properties of 30S ribosomal subunits from untreated and from colicin E3-treated (E3-30S) bacteria have been compared. Polyacrylamide gel electrophoresis of ribosomal proteins revealed no difference, but several studies indicated that the 16S RNA from E3-30S particles was modified. E3-16S RNA showed slightly increased resistance to heat-induced degradation and had a reduced (15 S) sedimentation coefficient on sucrose gradients. Fingerprint analyses of E3-16S RNA revealed that the 3′-terminus of the molecule had been deleted. It was concluded that a primary effect of colicin E3 is the activation of a highly specific RNase that degrades 30S ribosomal RNA in situ, and that the resulting fragment(s) are probably retained within the 30S particle.

« Previous | Next Article »Table of Contents

This Article

  1. PNAS 1971-05 vol. 68 no. 5 959-963
  1. » Abstract
  2. Full Text (PDF)
About Our New Site Design >>
Link: Advanced Search

This Week's Issue

  1. July 1, 2008, 105 (26)
  1. Current Issue
  1. Alert me to new issues of PNAS

Most