Translocation of Messenger RNA and “Accommodation” of fMet-tRNA

Abstract

Messenger RNA is moved a distance of approximately three nucleotides in the 5′ direction relative to the ribosome during the translocation of peptidyl-tRNA from the A to the P site. This movement is catalyzed by G factor and is dependent on the hydrolysis of GTP. In contrast, mRNA is not moved during the f₂-catalyzed hydrolysis of GTP that is involved in the activation of ribosome-bound fMet-tRNA. This second type of GTP-dependent reaction has been named “Accommodation”.

• GMP-PCP
• elongation factors
• initiation factors
• oligonucleotides
• ribosomes