Mercurated Dextran Column Chromatography for Fractionating Mononucleotides

  1. Dieter W. Gruenwedel and
  2. Joseph C. C. Fu
  1. 1Department of Food Sciences & Technology, University of California, Davis, Calif. 95616

Abstract

Mercurated dextran (polysaccharide) was found to fractionate mononucleotides according to their affinity for organomercurial Hg+. The nucleotides are released from the chromatographic column in the sequence CMP, AMP, GMP, and TMP when borate buffer of increasing pH is used as an eluent.

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  1. PNAS September 1, 1971 vol. 68 no. 9 2002-2005
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