Sedimentation Properties of Yeast Chromosomal DNA

  1. Thomas D. Petes and
  2. Walton L. Fangman
  1. 1Department of Genetics, University of Washington, Seattle, Wash. 98195

Abstract

Sedimentation analysis of nuclear DNA released from spheroplasts of the yeast Saccharomyces cerevisiae indicates that it has a number average molecular weight of 6.2 × 108. The chromosomal DNA molecules range in size from as small as 5 × 107 daltons to as large as 1.4 × 109 daltons. Based on these values and estimates of the total DNA content of the yeast nucleus, it is proposed that each yeast chromosome contains a single DNA duplex.

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  1. PNAS 1972-05 vol. 69 no. 5 1188-1191
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