Enzymatic Synthesis of Solid Phase-Bound DNA Sequences Corresponding to Specific Mammalian Genes

  1. Pal Venetianer* and
  2. Philip Leder
  1. 1Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20014

Abstract

With oligo(dT)-cellulose as primer, RNA-dependent DNA polymerase catalyzes the synthesis of cellulose-bound DNA that is complementary to mouse globin mRNA. The resulting cellulose-bound or solid phase complementary DNA hybridizes specifically with globin mRNA and permits the recovery of intact globin mRNA. This simple technique for the synthesis of solid phase-bound complementary DNA provides an additional and convenient method for the purification of specific genetic sequences.

Footnotes

  • ↵ * On leave from the Institute of Biochemistry, Biological Research Center, Szeged, Hungary.

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  1. PNAS 1974-10 vol. 71 no. 10 3892-3895
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