Latent DNA of Epstein-Barr Virus: Separation from High-Molecular-Weight Cell DNA in a Neutral Glycerol Gradient

  1. Akiko Tanaka and
  2. Meihan Nonoyama
  1. Department of Microbiology, University of Illinois Medical Centers, Chicago, Ill. 60612
  2. Department of Rush-Presbyterian-St. Luke's, University of Illinois Medical Centers, Chicago, Ill. 60612

Abstract

Epstein-Barr virus DNA in virus non-productive cells was separated from high-molecular-weight cell DNA by sedimentation through a neutral glycerol gradient after gentle lysis of cells by Pronase and Sarkosyl. The isolated Epstein-Barr virus DNA had a density of 1.716-1.717 g/cm3 in CsCl equilibrium centrifugation, which is very close to the virus DNA density of 1.718 g/cm3. The results indicated that the majority of Epstein-Barr virus DNA in virus nonproductive cells is not covalently integrated into high-molecular-weight cell DNA.

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  1. PNAS 1974-12 vol. 71 no. 12 4658-4661
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