Magnetic Circular Dichroism Studies XXV. A Preliminary Investigation of Microsomal Cytochromes*

  1. Peter M. Dolinger,
  2. Michael Kielczewski,
  3. James R. Trudell†,
  4. Günter Barth,
  5. Robert E. Linder,
  6. Edward Bunnenberg, and
  7. Carl Djerassi‡
  1. Department of Chemistry, Stanford University, Stanford, California 94305
  2. †Department of Anesthesia, Stanford University School of Medicine, Stanford, California 94305

Abstract

The application of magnetic circular dichroism as an optical probe for simultaneous identification and determination of at least two microsomal cytochromes is demonstrated. The assignments of the bands in the spectra of microsomal suspensions are made from the spectra of soluble preparations of cytochrome P-450 obtained from Pseudomonas putida and of cytochrome b 5 obtained from rat livers.

Footnotes

  • ↵ ‡ Author to whom correspondence should be addressed.

  • ↵ * For part XXIV, see Eckstein, H., Barth, G., Linder, R. E., Bunnenberg, E. & Djerassi, C. (1974) Liebig's Ann. Chem., in press.

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  1. PNAS 1974-02 vol. 71 no. 2 399-403
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