In Vitro Activation of the Transcription of araBAD Operon by araC Activator

  1. Nancy Lee,
  2. Gary Wilcox,
  3. William Gielow,
  4. John Arnold,
  5. Paul Cleary, and
  6. Ellis Englesberg
  1. 1Biochemistry & Molecular Biology Section, Department of Biological Sciences, University of California, Santa Barbara, Santa Barbara, Calif. 93106

Abstract

The transcription of araBAD operon requires araC activator and cyclic AMP. D-Fucose inhibits ara mRNA synthesis. Our results indicate that the positive control by araC activator is exerted at the level of transcription.

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