Localization of Replicating DNA of Simian Virus 40 in Monkey Kidney Cells

  1. Donald J. LeBlanc and
  2. Maxine F. Singer
  1. 1National Institute of Arthritis, Metabolism, and Digestive Diseases, National Institutes of Health, Bethesda, Maryland 20014

Abstract

Approximately 80-85% of the DNA synthesized by cells infected with simian virus 40 during a short pulse with [3H]thymidine is associated with host nuclear membrane components, as defined by the M-band technique. A substantial portion of this membrane-associated DNA has been shown to be viral specific by sedimentation studies and by DNA·DNA hybridization. Examination of DNA associated and unassociated with nuclear M-band by neutral sucrose gradient analysis suggested that while replicating DNA of simian virus 40 is attached to the nuclear membrane, mature component I DNA of simian virus 40 is released from the membrane upon completion of replication.

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  1. PNAS 1974-06 vol. 71 no. 6 2236-2240
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