Nucleotide Sequence Analysis of RNA Synthesized from Rabbit Globin Complementary DNA
- Raymond Poon*,
- Gary V. Paddock*,
- Howard Heindell*,
- Philip Whitcome*,
- Winston Salser*,
- Dan Kacian†,
- Arthur Bank‡,
- Roberto Gambino‡, and
- Francesco Ramirez‡
- *Department of Biology and Molecular Biology Institute, University of California at Los Angeles, California 90024
- †Institute of Cancer Research, Columbia University College of Physicians and Surgeons, New York, N.Y. 10032
- ‡Department of Medicine, Columbia University College of Physicians and Surgeons, New York, N.Y. 10032
- Department of Human Genetics and Development, Columbia University College of Physicians and Surgeons, New York, N.Y. 10032
Abstract
Rabbit globin complementary DNA made with RNA-dependent DNA polymerase (reverse transcriptase) was used as template for in vitro synthesis of 32P-labeled RNA. The sequences of the nucleotides in most of the fragments resulting from combined ribonuclease T1 and alkaline phosphatase digestion have been determined. Several fragments were long enough to fit uniquely with the α or β globin amino-acid sequences. These data demonstrate that the cDNA was copied from globin mRNA and contained no detectable contaminants.
