Identification and purification of the Lon+ (capR+) gene product, a DNA-binding protein

  1. Barbara A. Zehnbauer*,
  2. Edward C. Foley,
  3. Gordon W. Henderson, and
  4. Alvin Markovitz†
  1. Department of Microbiology, The University of Chicago, Chicago, Illinois 60637

Abstract

The polypeptide product of the lon (capR) gene was identified and partially purified from bacterial strains homozygous for the capR + or capR9 (ochre mutation) alleles cloned with pSC101. A 94,000-dalton polypeptide was identified as the lon (capR) gene product. Studies of binding to DNA cellulose columns and nitrocellulose filters indicate that the capR+ and capR9 proteins bind DNA.

Footnotes

  • ↵ * Present address: Department of Genetics, University of Wisconsin, Madison, WI 53706.

  • ↵ † To whom reprint requests should be addressed.

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  1. PNAS 1981-04 vol. 78 no. 4 2043-2047
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