Rapid processing of the carboxyl terminus of a trypanosome variant surface glycoprotein
- J D Bangs,
- D Hereld,
- J L Krakow,
- G W Hart, and
- P T Englund
Abstract
The variant surface glycoprotein of the parasite Trypanosoma brucei contains a glycolipid of unknown structure covalently attached to its COOH terminus. We have shown, by using metabolic labeling with [35S]methionine or [3H]myristic acid, precipitation with specific antibodies, and NaDodSO4/polyacrylamide gel electrophoresis, that this glycolipid is attached to the variant surface glycoprotein polypeptide within 1 min after its translation.
