Human immunodeficiency virus 1 tat protein binds trans-activation-responsive region (TAR) RNA in vitro
- C Dingwall,
- I Ernberg,
- M J Gait,
- S M Green,
- S Heaphy,
- J Karn,
- A D Lowe,
- M Singh,
- M A Skinner, and
- R Valerio
Abstract
tat, the trans-activator protein for human immunodeficiency virus 1 (HIV-1), has been expressed in Escherichia coli from synthetic genes. Purified tat binds specifically to HIV-1 trans-activation-responsive region (TAR) RNA in gel-retardation, filter-binding, and immunoprecipitation assays. tat does not bind detectably to antisense TAR RNA sequences, cellular mRNA sequences, variant TAR RNA sequences with altered stem-loop structures, or TAR DNA.
