Mobility of the maize suppressor-mutator element in transgenic tobacco cells

  1. P Masson and
  2. N V Fedoroff
  1. Department of Embryology, Carnegie Institution of Washington, Baltimore, MD 21210.

Abstract

Maize Suppressor-mutator (Spm) transposable elements have been introduced into tobacco cells and a visual assay for Spm activity has been developed using a bacterial beta-glucuronidase gene. The Spm element is mobile in tobacco and can trans-activate excision of a transposition-defective Spm (dSpm) element either from a different site on the same transforming Ti plasmid or from a second plasmid. An Spm element expressed from the stronger cauliflower mosaic virus 35S promoter trans-activates transposition of a dSpm element earlier after its introduction into tobacco cells than an element expressed from its own promoter.

« Previous | Next Article »Table of Contents

This Article

  1. PNAS 1989-04 vol. 86 no. 7 2219-2223
  1. » Abstract
  2. Full Text (PDF)

Classifications

About Our New Site Design >>
Link: Advanced Search

This Week's Issue

  1. July 22, 2008, 105 (29)
  1. Current Issue
  1. Alert me to new issues of PNAS

Most