Phylogeny, in situ hybridization service Sign up for PNAS Online eTocs
Link: Info for AuthorsLink: Editorial BoardLink: AboutLink: SubscribeLink: AdvertiseLink: ContactLink: Sitemap Link: PNAS Home
Proceedings of the National Academy of Sciences
Link: Current Issue "" Link: Archives "" Link: Online Submission ""  Link: Advanced Search



This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a colleague
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My File Cabinet
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bianchi, D. W.
Right arrow Articles by Latt, S. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bianchi, D. W.
Right arrow Articles by Latt, S. A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg  
What's this?

 Previous Article  | Table of Contents |  Next Article 

Proceedings of the National Academy of Sciences, Vol 87, 3279-3283, Copyright © 1990 by National Academy of Sciences

ARTICLE

Isolation of Fetal DNA from Nucleated Erythrocytes in Maternal Blood

DW Bianchi, AF Flint, MF Pizzimenti, JHM Knoll and SA Latt

Fetal nucleated cells within maternal blood represent a potential source of fetal genes obtainable by venipuncture. We used monoclonal antibody against the transferrin receptor (TfR) to identify nucleated erythrocytes in the peripheral blood of pregnant women. Candidate fetal cells from 19 pregnancies were isolated by flow sorting at 121/2-17 weeks gestation. The DNA in these cells was amplified for a 222-base-pair (bp) sequence present on the short arm of the Y chromosome as proof that the cells were derived from the fetus. The amplified DNA was compared with standardized DNA concentrations; 0.1-1 ng of fetal DNA was obtained in the 20-ml maternal samples. In 7/19 cases, a 222-bp band of amplified DNA was detected, consistent with the presence of male DNA in the isolated cells; 6/7 of these were confirmed as male pregnancies by karyotyping amniocytes. In the case of the female fetus, DNA prepared from samples at 32 weeks of gestation and cord blood at delivery also showed the presence of the Y chromosomal sequence, suggesting Y sequence mosaicism or translocation. In 10/12 cases where the 222-bp band was absent, the fetuses were female. Thus, we were successful in detecting the Y chromosomal sequence in 75% of the male-bearing pregnancies, demonstrating that it is possible to isolate fetal gene sequences from cells in maternal blood. Further refinement in methodology should increase sensitivity and facilitate noninvasive screening for fetal gene mutations.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg    What's this?


This article has been cited by other articles in HighWire Press-hosted journals:


Home page
 Obstet GynecolHome page
O. Lapaire, T. Volgmann, D. Huang, S. Hahn, W. Holzgreve, and Y. Xiao Zhong
Maternal Smoking: Effect on Circulating Cell-Free Fetal and Total DNA Levels in Maternal Plasma From the Second Trimester
Obstet. Gynecol., December 1, 2007; 110(6): 1358 - 1363.
[Abstract] [Full Text] [PDF]

Home page
 Ann Rheum DisHome page
I. C L Kremer Hovinga, M. Koopmans, H. J Baelde, E. de Heer, J. A Bruijn, and I. M Bajema
Tissue chimerism in systemic lupus erythematosus is related to injury
Ann Rheum Dis, December 1, 2007; 66(12): 1568 - 1573.
[Abstract] [Full Text] [PDF]

Home page
 Rheumatology (Oxford)Home page
I. C. L. Kremer Hovinga, M. Koopmans, E. de Heer, J. A. Bruijn, and I. M. Bajema
Chimerism in systemic lupus erythematosus--three hypotheses
Rheumatology, February 1, 2007; 46(2): 200 - 208.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
I. Stanghellini, R. Bertorelli, L. Capone, V. Mazza, C. Neri, A. Percesepe, and A. Forabosco
Quantitation of fetal DNA in maternal serum during the first trimester of pregnancy by the use of a DAZ repetitive probe
Mol. Hum. Reprod., September 1, 2006; 12(9): 587 - 591.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
Y.M. D. Lo
Recent Advances in Fetal Nucleic Acids in Maternal Plasma
J. Histochem. Cytochem., March 1, 2005; 53(3): 293 - 296.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
J. Choe, D. Hwang, K.-C. Kim, and Y.-M. Choi
Fetal Gender Determination and BclI Polymorphism Using Nucleated Erythrocytes in Maternal Blood
J. Histochem. Cytochem., March 1, 2005; 53(3): 323 - 327.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
C. Vernochet, S. M. Caucheteux, M.-C. Gendron, J. Wantyghem, and C. Kanellopoulos-Langevin
Affinity-Dependent Alterations of Mouse B Cell Development by Noninherited Maternal Antigen
Biol Reprod, February 1, 2005; 72(2): 460 - 469.
[Abstract] [Full Text] [PDF]

Home page
 Ann. N. Y. Acad. Sci.Home page
B. TABACK and D. S. B. HOON
Circulating Nucleic Acids and Proteomics of Plasma/Serum: Clinical Utility
Ann. N.Y. Acad. Sci., June 1, 2004; 1022(1): 1 - 8.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
M. Choolani, K. O'Donoghue, D. Talbert, S. Kumar, I. Roberts, E. Letsky, P. R. Bennett, and N. M. Fisk
Characterization of first trimester fetal erythroblasts for non-invasive prenatal diagnosis
Mol. Hum. Reprod., April 1, 2003; 9(4): 227 - 235.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
M. Imaizumi, A. Pritsker, P. Unger, and T. F. Davies
Intrathyroidal Fetal Microchimerism in Pregnancy and Postpartum
Endocrinology, January 1, 2002; 143(1): 247 - 253.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
G. Coata, F. Tilesi, M. Fizzotti, V. Lauro, L. Pennacchi, A. Tabilio, and G. C. Di Renzo
Prenatal Diagnosis of Genetic Abnormalities Using Fetal CD34+ Stem Cells in Maternal Circulation and Evidence They Do Not Affect Diagnosis in Later Pregnancies
Stem Cells, November 1, 2001; 19(6): 534 - 542.
[Abstract] [Full Text]

Home page
 Ann. N. Y. Acad. Sci.Home page
S. HOLDENRIEDER, P. STIEBER, H. BODENMULLER, M. BUSCH, J. V. PAWEL, A. SCHALHORN, D. NAGEL, and D. SEIDEL
Circulating Nucleosomes in Serum
Ann. N.Y. Acad. Sci., September 1, 2001; 945(1): 93 - 102.
[Abstract] [Full Text] [PDF]

Home page
 Ann. N. Y. Acad. Sci.Home page
D. W. BIANCHI and Y. M. D. LO
Fetomaternal Cellular and Plasma DNA Trafficking: The Yin and the Yang
Ann. N.Y. Acad. Sci., September 1, 2001; 945(1): 119 - 131.
[Abstract] [Full Text] [PDF]

Home page
 Ann. N. Y. Acad. Sci.Home page
A. V. LICHTENSTEIN, H. S. MELKONYAN, L. D. TOMEI, and S. R. UMANSKY
Circulating Nucleic Acids and Apoptosis
Ann. N.Y. Acad. Sci., September 1, 2001; 945(1): 239 - 249.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. A. Huie, M.-C. Cheung, M. O. Muench, B. Becerril, Y. W. Kan, and J. D. Marks
Antibodies to human fetal erythroid cells from a nonimmune phage antibody library
PNAS, February 27, 2001; 98(5): 2682 - 2687.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
C. Wei, D. N. Saller, and J.W.H. Sutherland
Detection and Quantification by Homogeneous PCR of Cell-free Fetal DNA in Maternal Plasma
Clin. Chem., February 1, 2001; 47(2): 336 - 338.
[Full Text] [PDF]

Home page
 Clin. Chem.Home page
Y.M. D. Lo, T. K. Lau, L. Y.S. Chan, T. N. Leung, and A. M.Z. Chang
Quantitative Analysis of the Bidirectional Fetomaternal Transfer of Nucleated Cells and Plasma DNA
Clin. Chem., September 1, 2000; 46(9): 1301 - 1309.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
J.-Y. Wang, D.K. Zhen, M.E. Zilberstein, V.M. Falco, and D.W. Bianchi
Non-invasive exclusion of fetal aneuploidy in an at-risk couple with a balanced translocation
Mol. Hum. Reprod., February 1, 2000; 6(2): 103 - 106.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
C. Troeger, X.Y. Zhong, R. Burgemeister, S. Minderer, S. Tercanli, W. Holzgreve, and S. Hahn
Approximately half of the erythroblasts in maternal blood are of fetal origin
Mol. Hum. Reprod., December 1, 1999; 5(12): 1162 - 1165.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
F. V. Alvarez, J. Olander, D. Crimmins, B. Prieto, A. Paz, R. Alonso, S. Porter, J. Hess, R. D. Crist, Y. Landt, et al.
Development, Characterization, and Use of Monoclonal Antibodies Made to Antigens Expressed on the Surface of Fetal Nucleated Red Blood Cells
Clin. Chem., September 1, 1999; 45(9): 1614 - 1620.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
Y.M. D. Lo, T. N. Leung, M. S.C. Tein, I. L. Sargent, J. Zhang, T. K. Lau, C. J. Haines, and C. W.G. Redman
Quantitative Abnormalities of Fetal DNA in Maternal Serum in Preeclampsia
Clin. Chem., February 1, 1999; 45(2): 184 - 188.
[Abstract] [Full Text] [PDF]

Home page
 NEJMHome page
Y.M. D. Lo, N. M. Hjelm, C. Fidler, I. L. Sargent, M. F. Murphy, P. F. Chamberlain, P. M.K. Poon, C. W.G. Redman, and J. S. Wainscoat
Prenatal Diagnosis of Fetal RhD Status by Molecular Analysis of Maternal Plasma
N. Engl. J. Med., December 10, 1998; 339(24): 1734 - 1738.
[Abstract] [Full Text] [PDF]

Home page
 Ann. N. Y. Acad. Sci.Home page
M. I. EVANS, J. E. O'BRIEN, and G. CRITCHFIELD
Detection of Anomalies: Alternatives to Ultrasound
Ann. N.Y. Acad. Sci., June 18, 1998; 847(1): 191 - 199.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
M.-T. Little, S. Langlois, R. D. Wilson, and P. M. Lansdorp
Frequency of Fetal Cells in Sorted Subpopulations of Nucleated Erythroid and CD34+ Hematopoietic Progenitor Cells From Maternal Peripheral Blood
Blood, April 1, 1997; 89(7): 2347 - 2358.
[Abstract] [Full Text] [PDF]

Home page
 NEJMHome page
M. E. D'Alton and A. H. DeCherney
Prenatal Diagnosis
N. Engl. J. Med., January 14, 1993; 328(2): 114 - 120.
[Full Text]