Recombination walking: genetic selection of clones from pooled libraries of yeast artificial chromosomes by homologous recombination

  1. A M Miller,
  2. E A Savinelli,
  3. S M Couture,
  4. G M Hannigan,
  5. Z Han,
  6. R F Selden, and
  7. D A Treco
  1. Transkaryotic Therapies, Inc., Cambridge, MA 02139.

Abstract

Recombination walking is based on the genetic selection of specific human clones from a yeast artificial chromosome (YAC) library by homologous recombination. The desired clone is selected from a pooled (unordered) YAC library, eliminating labor-intensive steps typically used in organizing and maintaining ordered YAC libraries. Recombination walking represents an efficient approach to library screening and is well suited for chromosome-walking approaches to the isolation of genes associated with common diseases.

« Previous | Next Article »Table of Contents

This Article

  1. PNAS September 1, 1993 vol. 90 no. 17 8118-8122
  1. » Abstract
  2. Full Text (PDF)

Classifications

About Our New Site Design >>
Link: Advanced Search

This Week's Issue

  1. July 22, 2008, 105 (29)
  1. Current Issue
  1. Alert me to new issues of PNAS

Most