Accumulation of plasma cells in atherosclerotic lesions of Watanabe heritable hyperlipidemic rabbits

  1. Y Sohma,
  2. H Sasano,
  3. R Shiga,
  4. S Saeki,
  5. T Suzuki,
  6. H Nagura,
  7. M Nose, and
  8. T Yamamoto
  1. Tohoku University Gene Research Center, Tohoku University, Sendai, Japan.

Abstract

By screening a cDNA library constructed from aortic total RNA derived from Watanabe heritable hyperlipidemic (WHHL) rabbits by differential hybridization, we have obtained a cDNA encoding the kappa light chain of immunoglobulin. Northern blot analysis of total RNA prepared from aortas of WHHL and normal rabbits of various ages revealed that this light-chain mRNA accumulates gradually with age in aortas in WHHL rabbits. Northern blotting and in situ hybridization with an antisense oligonucleotide specific to rabbit immunoglobulin gamma heavy-chain mRNA also detected accumulation of this heavy-chain mRNA in advanced lesions of WHHL rabbit aortas. Moreover, immunohistochemical and electron microscopic analyses demonstrated the presence of plasma cells in the atherosclerotic lesions.

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  1. PNAS May 23, 1995 vol. 92 no. 11 4937-4941
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