Parallel human genome analysis: microarray-based expression monitoring of 1000 genes

  1. M Schena,
  2. D Shalon,
  3. R Heller,
  4. A Chai,
  5. P O Brown, and
  6. R W Davis
  1. Department of Biochemistry, Beckman Center, Stanford University Medical Center, CA 94305, USA. schena@cmgm.stanford.edu

Abstract

Microarrays containing 1046 human cDNAs of unknown sequence were printed on glass with high-speed robotics. These 1.0-cm2 DNA "chips" were used to quantitatively monitor differential expression of the cognate human genes using a highly sensitive two-color hybridization assay. Array elements that displayed differential expression patterns under given experimental conditions were characterized by sequencing. The identification of known and novel heat shock and phorbol ester-regulated genes in human T cells demonstrates the sensitivity of the assay. Parallel gene analysis with microarrays provides a rapid and efficient method for large-scale human gene discovery.

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  1. PNAS October 1, 1996 vol. 93 no. 20 10614-10619
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