Biosynthesis of vitamin B12: Factor IV, a new intermediate in the anaerobic pathway

  1. A. Ian Scott*,†,
  2. Neal J. Stolowich*,
  3. Jianji Wang*,
  4. Oliver Gawatz‡,
  5. Erwin Fridrich‡, and
  6. Gerhard Müller‡
  1. *Center for Biological NMR, Department of Chemistry, Texas A&M University, College Station, TX 77843-3255; and ‡Institute für Organische Chemie, Biochemie und Isotopenforschung, Universität Stuttgart, Azenbergstrasse 18, D-7000 Stuttgart 1, Germany

Abstract

The structure of a novel tetradehydrocorrin, factor IV, isolated from Propionibacterium shermanii has been established by multidimensional NMR spectroscopy. Incorporation of radiolabeled factor IV into cobyrinic acid established the biointermediacy of this cobalt complex, whose structure has implications for the mechanisms of the anaerobic pathway to B12.

Footnotes

  • ↵ † To whom reprint requests should be addressed. e-mail: Scott{at}chemvx.tamu.edu.

  • D. H. R. Barton, Texas A&M University, College Station, TX

  • Abbreviations: ALA, δ-aminolevulinic acid; AdoMet, S-adenosylmethionine.

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  1. PNAS December 10, 1996 vol. 93 no. 25 14316-14319
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