This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a colleague Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Add to My File Cabinet Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via ISI Web of Science (29) Citing Articles via Google Scholar Google Scholar Articles by Ruiz-Echevarría, M. J. Articles by Peltz, S. W. Search for Related Content PubMed PubMed Citation Articles by Ruiz-Echevarría, M. J. Articles by Peltz, S. W. Social Bookmarking                What's this?

 Previous Article  | Table of Contents |  Next Article 

Vol. 95, Issue 15, 8721-8726, July 21, 1998

Genetics
The Upf3 protein is a component of the surveillance complex that monitors both translation and mRNA turnover and affects viral propagation

Maria J. Ruiz-Echevarría^*,, Jason M. Yasenchak^*,,, Xia Han^*,, Jonathan D. Dinman^*,, and Stuart W. Peltz^*,,§,¶

^* Department of Molecular Genetics and Microbiology and  Graduate Program in Molecular Biosciences at University of Medicine and Dentistry of New Jersey/Rutgers Universities, Robert Wood Johnson Medical School-University of Medicine and Dentistry of New Jersey, and ^§ The Cancer Institute of New Jersey, 675 Hoes Lane, Piscataway NJ 08854

Edited by Fred Sherman, University of Rochester School of Medicine, Rochester, NY, and approved May 28, 1998 (received for review March 30, 1998)

The nonsense-mediated mRNA decay pathway functions to degrade aberrant mRNAs that contain premature translation termination codons. In Saccharomyces cerevisiae, the Upf1, Upf2, and Upf3 proteins have been identified as trans-acting factors involved in this pathway. Recent results have demonstrated that the Upf proteins may also be involved in maintaining the fidelity of several aspects of the translation process. Certain mutations in the UPF1 gene have been shown to affect the efficiency of translation termination at nonsense codons and/or the process of programmed 1 ribosomal frameshifting used by viruses to control their gene expression. Alteration of programmed frameshift efficiencies can affect virus assembly leading to reduced viral titers or elimination of the virus. Here we present evidence that the Upf3 protein also functions to regulate programmed 1 frameshift efficiency. A upf3- strain demonstrates increased sensitivity to the antibiotic paromomycin and increased programmed 1 ribosomal frameshift efficiency resulting in loss of the M₁ virus. Based on these observations, we hypothesize that the Upf proteins are part of a surveillance complex that functions to monitor translational fidelity and mRNA turnover.

Copyright © 1998 by The National Academy of Sciences  0027-8424/98/958721-6$2.00/0
CiteULike    Complore    Connotea    Del.icio.us    Digg    What's this?

This article has been cited by other articles in HighWire Press-hosted journals:

				J. W. HARGER and J. D. DINMAN Evidence against a direct role for the Upf proteins in frameshifting or nonsense codon readthrough RNA, November 18, 2004; 10(11): 1721 - 1729. [Abstract] [Full Text] [PDF]

				A. Meskauskas, J. L. Baxter, E. A. Carr, J. Yasenchak, J. E. G. Gallagher, S. J. Baserga, and J. D. Dinman Delayed rRNA Processing Results in Significant Ribosome Biogenesis and Functional Defects Mol. Cell. Biol., March 1, 2003; 23(5): 1602 - 1613. [Abstract] [Full Text] [PDF]

				K. S. Rajavel and E. F. Neufeld Nonsense-Mediated Decay of Human HEXA mRNA Mol. Cell. Biol., August 15, 2001; 21(16): 5512 - 5519. [Abstract] [Full Text] [PDF]

				F. He and A. Jacobson Upf1p, Nmd2p, and Upf3p Regulate the Decapping and Exonucleolytic Degradation of both Nonsense-Containing mRNAs and Wild-Type mRNAs Mol. Cell. Biol., March 1, 2001; 21(5): 1515 - 1530. [Abstract] [Full Text]

				Y.-G. Kim, S. Maas, and A. Rich Comparative mutational analysis of cis-acting RNA signals for translational frameshifting in HIV-1 and HTLV-2 Nucleic Acids Res., March 1, 2001; 29(5): 1125 - 1131. [Abstract] [Full Text] [PDF]

				G. Serin, A. Gersappe, J. D. Black, R. Aronoff, and L. E. Maquat Identification and Characterization of Human Orthologues to Saccharomyces cerevisiae Upf2 Protein and Upf3 Protein (Caenorhabditis elegans SMG-4) Mol. Cell. Biol., January 1, 2001; 21(1): 209 - 223. [Abstract] [Full Text]

				A. B. Maderazo, F. He, D. A. Mangus, and A. Jacobson Upf1p Control of Nonsense mRNA Translation Is Regulated by Nmd2p and Upf3p Mol. Cell. Biol., July 1, 2000; 20(13): 4591 - 4603. [Abstract] [Full Text]

				B. Das, Z. Guo, P. Russo, P. Chartrand, and F. Sherman The Role of Nuclear Cap Binding Protein Cbc1p of Yeast in mRNA Termination and Degradation Mol. Cell. Biol., April 15, 2000; 20(8): 2827 - 2838. [Abstract] [Full Text]

				J. D. Lopinski, J. D. Dinman, and J. A. Bruenn Kinetics of Ribosomal Pausing during Programmed -1 Translational Frameshifting Mol. Cell. Biol., February 15, 2000; 20(4): 1095 - 1103. [Abstract] [Full Text]

				G. M. Wilson and G. Brewer Slip-Sliding the Frame: Programmed -1 Frameshifting on Eukaryotic Transcripts Genome Res., May 1, 1999; 9(5): 393 - 394. [Full Text]

				S. W. Peltz, A. B. Hammell, Y. Cui, J. Yasenchak, L. Puljanowski, and J. D. Dinman Ribosomal Protein L3 Mutants Alter Translational Fidelity and Promote Rapid Loss of the Yeast Killer Virus Mol. Cell. Biol., January 1, 1999; 19(1): 384 - 391. [Abstract] [Full Text] [PDF]

Current Issue | Archives | Online Submission | Info for Authors | Editorial Board | About Subscribe | Advertise | Contact | Site Map Copyright © 1998 by the National Academy of Sciences