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Vol. 95, Issue 23, 13726-13731, November 10, 1998 (alkaline phosphatase/embryoid body/embryonic stem
cell/embryonic germ cell)
Departments of * Gynecology and Obstetrics and Contributed by John W. Littlefield, September 29, 1998
Human pluripotent stem cells would be invaluable for in
vitro studies of aspects of human embryogenesis. With the goal
of establishing pluripotent stem cell lines, gonadal ridges and
mesenteries containing primordial germ cells (PGCs, 5-9 weeks
postfertilization) were cultured on mouse STO fibroblast feeder layers
in the presence of human recombinant leukemia inhibitory factor, human
recombinant basic fibroblast growth factor, and forskolin. Initially,
single PGCs in culture were visualized by alkaline phosphatase activity staining. Over a period of 7-21 days, PGCs gave rise to large multicellular colonies resembling those of mouse pluripotent stem cells
termed embryonic stem and embryonic germ (EG) cells. Throughout the
culture period most cells within the colonies continued to be alkaline
phosphatase-positive and tested positive against a panel of five
immunological markers (SSEA-1, SSEA-3, SSEA-4, TRA-1-60, and
TRA-1-81) that have been used routinely to characterize embryonic stem
and EG cells. The cultured cells have been continuously passaged and
found to be karyotypically normal and stable. Both XX and XY cell
cultures have been obtained. Immunohistochemical analysis of embryoid
bodies collected from these cultures revealed a wide variety of
differentiated cell types, including derivatives of all three embryonic
germ layers. Based on their origin and demonstrated properties, these
human PGC-derived cultures meet the criteria for pluripotent stem cells
and most closely resemble EG cells.
Copyright © 1998 by The National Academy of Sciences 0027-8424/98/9513726-6$2.00/0
Developmental Biology
Derivation of pluripotent stem cells from cultured human
primordial germ cells
,
,,¶
Physiology,
Johns Hopkins University School of Medicine, Baltimore, MD 21287;
Kimmel Cancer Institute, Jefferson Medical College,
Philadelphia, PA 19107; and § Department of Gynecology and
Obstetrics, Johns Hopkins Bayview Hospital, Baltimore, MD 21224
¶
To whom reprint requests should be addressed at: Johns
Hopkins University School of Medicine, Department of Gynecology and Obstetrics, 600 North Wolfe Street, Park Building B2-210, Baltimore, MD
21287. e-mail: gearhart{at}welchlink.welch.jhu.edu.
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|
|
|
|
|
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|
|
|
|
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|
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|
|
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|
|
|
|
|
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|
|
|
|
 |
|
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|
|
|
|
 |
|
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|
|
|
|
|
|
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|
|
|
|
 |
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|
|
|
|
|
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|
|
|
|
 |
|
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|
|
|
|
 |
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
 |
|
 E. M. Meslin and S. E. Martin From Stem to Stern: A Brief Review of Ethical Issues in the Stem Cell Debate NeoReviews, July 1, 2003; 4(7): e187 - 193. [Full Text] [PDF]
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D. A. Kerr, J. Llado, M. J |
