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Vol. 96, Issue 1, 11-13, January 5, 1999

Applied Physical Sciences / Biophysics
A microfabricated device for sizing and sorting DNA molecules

Department of Applied Physics, California Institute of Technology, Pasadena, CA 91125

Communicated by John D. Baldeschwieler, California Institute of Technology, Pasadena, CA, October 26, 1998 (received for review August 28, 1998)

We have demonstrated a microfabricated single-molecule DNA sizing device. This device does not depend on mobility to measure molecule size, is 100 times faster than pulsed-field gel electrophoresis, and has a resolution that improves with increasing DNA length. It also requires a million times less sample than pulsed-field gel electrophoresis and has comparable resolution for large molecules. Here we describe the fabrication and use of the single-molecule DNA sizing device for sizing and sorting DNA restriction digests and ladders spanning 2-200 kbp.

Copyright © 1999 by The National Academy of Sciences  0027-8424/99/9611-3$2.00/0
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