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Vol. 96, Issue 1, 79-84, January 5, 1999
Department of Pharmacology, Joan and Sanford I. Weill Medical
College of Cornell University, 1300 York Avenue, New York, NY 10021
Edited by Robert J. Lefkowitz, Duke University Medical Center,
Durham, NC, and approved November 6, 1998 (received for review October 13, 1998)
Mammals have nine differentially regulated isoforms of G
protein-responsive transmembrane-spanning adenylyl cyclases. We now describe the existence of a distinct class of mammalian adenylyl cyclase that is soluble and insensitive to G protein or Forskolin regulation. Northern analysis indicates the gene encoding soluble adenylyl cyclase (sAC) is preferentially expressed in testis. As
purified from rat testis cytosol, the active form of sAC appears to be
a fragment derived from the full-length protein, suggesting a
proteolytic mechanism for sAC activation. The two presumptive catalytic
domains of sAC are closely related to cyanobacterial adenylyl cyclases,
providing an evolutionary link between bacterial and mammalian
signaling molecules.
Copyright © 1999 by The National Academy of Sciences 0027-8424/99/9679-6$2.00/0
Biochemistry
Cytosolic adenylyl cyclase defines a unique signaling molecule
in mammals
*
J.B. and M.L.S. contributed equally to this work.
To whom reprint requests should be addressed. e-mail:
llevin{at}mail.med.cornell.edu.
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