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Vol. 96, Issue 23, 13086-13090, November 9, 1999

Biochemistry
 The dimensions of the protein import channels in the outer and inner mitochondrial membranes

Michael P. Schwartz and Andreas Matouschek*

Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, 2153 Sheridan Road, Evanston, IL 60208-3500

Communicated by Gottfried Schatz, University of Basel, Basel, Switzerland, September 2, 1999 (received for review May 28, 1999)

Most mitochondrial proteins are imported into mitochondria through transmembrane channels composed largely, and perhaps exclusively, of proteins. We have determined the effective internal diameter of the protein import channel in the mitochondrial outer membrane to be between 20 Å and 26 Å during translocation. The diameter of the import channel in the inner membrane is smaller than the diameter of the outer membrane import channel. These results were obtained by measuring the effect of rigid steric bulk introduced into precursor proteins on import.

*   To whom reprint requests should be addressed. E-mail: matouschek{at}nwu.edu.

Copyright © 1999 by The National Academy of Sciences  0027-8424/99/9613086-5$2.00/0
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