DNA replication in vertebrates requires a homolog of the Cdc7 protein kinase
- *Howard Hughes Medical Institute and Department of Pharmacology, University of Colorado Health Sciences Center, Box C-236, Denver, CO 80262; and †Department of Genetics and Development and Department of Dermatology, Columbia University, New York, NY 10032
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Communicated by Joan V. Ruderman, Harvard Medical School, Boston, MA (received for review October 13, 1998)
Abstract
CDC7 is an essential gene required for DNA replication in Saccharomyces cerevisiae. Cdc7p homologs have recently been identified in vertebrates, but their role in DNA replication has not yet been addressed. Here we show that antibodies to the Xenopus laevis homolog, xCdc7, interfere with DNA replication in vivo in developing embryos and in vitro in cycling egg extracts. We also demonstrate cell cycle-dependent association of xCdc7 with the Mcm complex, which binds to replication origins and also is required for DNA synthesis. Taken together, these data indicate that the function of xCdc7 is conserved from fungi to vertebrates. xCdc7 protein accumulates after stimulation of resting oocytes with progesterone, suggesting a molecular explanation for previous observations that the development of the capacity for DNA replication requires protein synthesis late in meiosis I.
