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* Department of Pathology and Infectious Diseases and Inflammation
Program, Departments of Edited by Peter Palese, Mount Sinai School of Medicine, New York,
NY, and approved July 11, 2000 (received for review May 1, 2000)
The relationship between hantaviruses and their reservoir hosts is
not well understood. We successfully passaged a mouse-adapted strain of
Sin Nombre virus from deer mice (Peromyscus maniculatus) by i.m. inoculation of 4- to 6-wk-old deer mouse pups. After
inoculation with 5 ID50, antibodies to the nucleocapsid (N)
antigen first became detectable at 14 d whereas neutralizing
antibodies were detectable by 7 d. Viral N antigen first began to
appear in heart, lung, liver, spleen, and/or kidney by 7 d,
whereas viral RNA was present in those tissues as well as in thymus,
salivary gland, intestine, white fat, and brown fat. By 14 d
nearly all tissues examined displayed both viral RNA and N antigen. We
noted no consistent histopathologic changes associated with infection,
even when RNA load was high. Viral RNA titers peaked on 21 d in
most tissues, then began to decline by 28 d. Infection persisted
for at least 90 d. The RNA titers were highest in heart, lung, and
brown fat. Deer mice can be experimentally infected with Sin Nombre
virus, which now allows provocative examination of the virus-host
relationship. The prominent involvement of heart, lung, and brown fat
suggests that these sites may be important tissues for early virus
replication or for maintenance of the virus in nature.
Microbiology
Experimental infection model for Sin Nombre hantavirus in the
deer mouse (Peromyscus maniculatus)
,
,,§,¶
Cell Biology and Physiology,
Biology, and § Molecular Genetics and
Microbiology, University of New Mexico School of Medicine, 915 Camino
de Salud NE, Albuquerque, NM 87131
¶
To whom reprint requests should be addressed at:
Department of Pathology, University of New Mexico School of Medicine,
Albuquerque, NM 87131. E-mail: bhjelle{at}salud.unm.edu.
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