This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a colleague Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Add to My File Cabinet Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via ISI Web of Science (29) Citing Articles via Google Scholar Google Scholar Articles by Teichmann, M. Articles by Roeder, R. G. Search for Related Content PubMed PubMed Citation Articles by Teichmann, M. Articles by Roeder, R. G. Social Bookmarking                What's this?

 Previous Article  | Table of Contents |  Next Article 

Biochemistry
A stable complex of a novel transcription factor IIB- related factor, human TFIIIB50, and associated proteins mediate selective transcription by RNA polymerase III of genes with upstream promoter elements

Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, 1230 York Avenue, New York, NY 10021

Contributed by Robert G. Roeder, October 31, 2000

Transcription factor IIIB (TFIIIB) is directly involved in transcription initiation by RNA polymerase III in eukaryotes. Yeast contain a single TFIIIB activity that is comprised of the TATA-binding protein (TBP), TFIIB-related factor 1 (BRF1), and TFIIIB'', whereas two distinct TFIIIB activities, TFIIIB- and TFIIIB-, have been described in human cells. Human TFIIIB- is required for transcription of genes with internal promoter elements, and contains TBP, a TFIIIB'' homologue (TFIIIB150), and a BRF1 homologue (TFIIIB90), whereas TFIIIB- is required for transcription of genes with promoter elements upstream of the initiation site. Here we describe the identification, cloning, and characterization of TFIIIB50, a novel homologue of TFIIB and TFIIIB90. TFIIIB50 and tightly associated factors, along with TBP and TFIIIB150, reconstitute human TFIIIB- activity. Thus, higher eukaryotes, in contrast to the yeast Saccharomyces cerevisiae, have evolved two distinct TFIIB-related factors that mediate promoter selectivity by RNA polymerase III.

CiteULike    Complore    Connotea    Del.icio.us    Digg    What's this?

This article has been cited by other articles in HighWire Press-hosted journals:

				H. Dumay-Odelot, C. Marck, S. Durrieu-Gaillard, O. Lefebvre, S. Jourdain, M. Prochazkova, A. Pflieger, and M. Teichmann Identification, Molecular Cloning, and Characterization of the Sixth Subunit of Human Transcription Factor TFIIIC J. Biol. Chem., June 8, 2007; 282(23): 17179 - 17189. [Abstract] [Full Text] [PDF]

				X. Liu and X. F. S. Zheng Endoplasmic Reticulum and Golgi Localization Sequences for Mammalian Target of Rapamycin Mol. Biol. Cell, March 1, 2007; 18(3): 1073 - 1082. [Abstract] [Full Text] [PDF]

				M. SZYMANSKI and J. BARCISZEWSKI RNA regulation in mammals. Ann. N.Y. Acad. Sci., May 1, 2006; 1067: 461 - 468. [Abstract] [Full Text] [PDF]

				J. A. Fairley, T. Kantidakis, N. S. Kenneth, R. V. Intine, R. J. Maraia, and R. J. White Human La is found at RNA polymerase III-transcribed genes in vivo PNAS, December 20, 2005; 102(51): 18350 - 18355. [Abstract] [Full Text] [PDF]

				A. Saxena, B. Ma, L. Schramm, and N. Hernandez Structure-Function Analysis of the Human TFIIB-Related Factor II Protein Reveals an Essential Role for the C-Terminal Domain in RNA Polymerase III Transcription Mol. Cell. Biol., November 1, 2005; 25(21): 9406 - 9418. [Abstract] [Full Text] [PDF]

				A. A. Gridasova and R. W. Henry The p53 Tumor Suppressor Protein Represses Human snRNA Gene Transcription by RNA Polymerases II and III Independently of Sequence-Specific DNA Binding Mol. Cell. Biol., April 15, 2005; 25(8): 3247 - 3260. [Abstract] [Full Text] [PDF]

				S. Weser, C. Gruber, H. M. Hafner, M. Teichmann, R. G. Roeder, K. H. Seifart, and W. Meissner Transcription Factor (TF)-like Nuclear Regulator, the 250-kDa Form of Homo sapiens TFIIIB'', Is an Essential Component of Human TFIIIC1 Activity J. Biol. Chem., June 25, 2004; 279(26): 27022 - 27029. [Abstract] [Full Text] [PDF]

				X. Zhao and W. Herr Role of the Inhibitory DNA-Binding Surface of Human TATA-Binding Protein in Recruitment of Human TFIIB Family Members Mol. Cell. Biol., November 15, 2003; 23(22): 8152 - 8160. [Abstract] [Full Text] [PDF]

				C. S. Hinkley, H. A. Hirsch, L. Gu, B. LaMere, and R. W. Henry The Small Nuclear RNA-activating Protein 190 Myb DNA Binding Domain Stimulates TATA Box-binding Protein-TATA Box Recognition J. Biol. Chem., May 9, 2003; 278(20): 18649 - 18657. [Abstract] [Full Text] [PDF]

				T. Lagrange, M.-A. Hakimi, D. Pontier, F. Courtois, J. P. Alcaraz, D. Grunwald, E. Lam, and S. Lerbs-Mache Transcription Factor IIB (TFIIB)-Related Protein (pBrp), a Plant-Specific Member of the TFIIB-Related Protein Family Mol. Cell. Biol., May 1, 2003; 23(9): 3274 - 3286. [Abstract] [Full Text] [PDF]

				S. Weser, J. Riemann, K. H. Seifart, and W. Meissner Assembly and isolation of intermediate steps of transcription complexes formed on the human 5S rRNA gene Nucleic Acids Res., May 1, 2003; 31(9): 2408 - 2416. [Abstract] [Full Text] [PDF]

				Y. Huang, E. McGillicuddy, M. Weindel, S. Dong, and R. J. Maraia The fission yeast TFIIB-related factor limits RNA polymerase III to a TATA-dependent pathway of TBP recruitment Nucleic Acids Res., April 15, 2003; 31(8): 2108 - 2116. [Abstract] [Full Text] [PDF]

				S. Jin, M. Kalkum, M. Overholtzer, A. Stoffel, B. T. Chait, and A. J. Levine CIAP1 and the serine protease HTRA2 are involved in a novel p53-dependent apoptosis pathway in mammals Genes & Dev., February 1, 2003; 17(3): 359 - 367. [Abstract] [Full Text] [PDF]

				P. Hu, S. Wu, Y. Sun, C.-C. Yuan, R. Kobayashi, M. P. Myers, and N. Hernandez Characterization of Human RNA Polymerase III Identifies Orthologues for Saccharomyces cerevisiae RNA Polymerase III Subunits Mol. Cell. Biol., November 15, 2002; 22(22): 8044 - 8055. [Abstract] [Full Text] [PDF]

				B. Ma and N. Hernandez Redundant Cooperative Interactions for Assembly of a Human U6 Transcription Initiation Complex Mol. Cell. Biol., November 15, 2002; 22(22): 8067 - 8078. [Abstract] [Full Text] [PDF]

				L. Schramm and N. Hernandez Recruitment of RNA polymerase III to its target promoters Genes & Dev., October 15, 2002; 16(20): 2593 - 2620. [Full Text] [PDF]

				P. Cabart and S. Murphy Assembly of Human Small Nuclear RNA Gene-specific Transcription Factor IIIB Complex de Novo On and Off Promoter J. Biol. Chem., July 19, 2002; 277(30): 26831 - 26838. [Abstract] [Full Text] [PDF]

				A. Ishiguro, G. A. Kassavetis, and E. P. Geiduschek Essential Roles of Bdp1, a Subunit of RNA Polymerase III Initiation Factor TFIIIB, in Transcription and tRNA Processing Mol. Cell. Biol., May 15, 2002; 22(10): 3264 - 3275. [Abstract] [Full Text] [PDF]

				W. Meissner, R. Thomae, and K. H. Seifart The Activity of Transcription Factor IIIC1 Is Impaired during Differentiation of F9 Cells J. Biol. Chem., February 22, 2002; 277(9): 7148 - 7156. [Abstract] [Full Text] [PDF]

				P. Cabart and S. Murphy BRFU, a TFIIB-like Factor, Is Directly Recruited to the TATA-box of Polymerase III Small Nuclear RNA Gene Promoters through Its Interaction with TATA-binding Protein J. Biol. Chem., November 9, 2001; 276(46): 43056 - 43064. [Abstract] [Full Text] [PDF]

				M. Hamada, Y. Huang, T. M. Lowe, and R. J. Maraia Widespread Use of TATA Elements in the Core Promoters for RNA Polymerases III, II, and I in Fission Yeast Mol. Cell. Biol., October 15, 2001; 21(20): 6870 - 6881. [Abstract] [Full Text] [PDF]

				T. E. Cloutier, M. D. Librizzi, A. K. M. M. Mollah, M. Brenowitz, and I. M. Willis Kinetic trapping of DNA by transcription factor IIIB PNAS, July 24, 2001; (2001) 161292298. [Abstract] [Full Text] [PDF]

				Y. Huang and R. J. Maraia Comparison of the RNA polymerase III transcription machinery in Schizosaccharomyces pombe, Saccharomyces cerevisiae and human Nucleic Acids Res., July 1, 2001; 29(13): 2675 - 2690. [Abstract] [Full Text] [PDF]

				S. S. Chong, P. Hu, and N. Hernandez Reconstitution of Transcription from the Human U6 Small Nuclear RNA Promoter with Eight Recombinant Polypeptides and a Partially Purified RNA Polymerase III Complex J. Biol. Chem., June 1, 2001; 276(23): 20727 - 20734. [Abstract] [Full Text] [PDF]

				K. Eichhorn and S. P. Jackson A Role for TAF3B2 in the Repression of Human RNA Polymerase III Transcription in Nonproliferating Cells J. Biol. Chem., June 8, 2001; 276(24): 21158 - 21165. [Abstract] [Full Text] [PDF]

				N. Hernandez Small Nuclear RNA Genes: a Model System to Study Fundamental Mechanisms of Transcription J. Biol. Chem., July 13, 2001; 276(29): 26733 - 26736. [Full Text] [PDF]

				T. E. Cloutier, M. D. Librizzi, A. K. M. M. Mollah, M. Brenowitz, and I. M. Willis Kinetic trapping of DNA by transcription factor IIIB PNAS, August 14, 2001; 98(17): 9581 - 9586. [Abstract] [Full Text] [PDF]

Current Issue | Archives | Online Submission | Info for Authors | Editorial Board | About Subscribe | Advertise | Contact | Site Map Copyright © 2000 by the National Academy of Sciences