A family of chromatin remodeling factors related to Williams syndrome transcription factor

A family of chromatin remodeling factors related to Williams syndrome transcription factor

^*The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104; ^†Laval University Cancer Research Center, Hôtel-Dieu de Québec, 11 côte du Palais, Quebec City, Qc G1R 2J6, Canada; ^‡Laboratory of Genetics, National Institute of Aging, National Institute of Health, Gerontology Research Center, 5600 Nathan Shock Drive, Baltimore, MD 21224; and ^§Department of Molecular Biology, Human Genome Sciences Incorporated, 9410 Key West Avenue, Rockville, MD 20874

Edited by Roger D. Kornberg, Stanford University School of Medicine, Stanford, CA, and approved December 6, 1999 (received for review October 12, 1999)

Abstract

Chromatin remodeling complexes have been implicated in the disruption or reformation of nucleosomal arrays resulting in modulation of transcription, DNA replication, and DNA repair. Here we report the isolation of WCRF, a new chromatin-remodeling complex from HeLa cells. WCRF is composed of two subunits, WCRF135, the human homolog of Drosophila ISWI, and WCRF180, a protein related to the Williams syndrome transcription factor. WCRF180 is a member of a family of proteins sharing a putative heterochromatin localization domain, a PHD finger, and a bromodomain, prevalent in factors involved in regulation of chromatin structure.

Footnotes

↵ ¶ To whom reprint requests should be addressed. E-mail: shiekhattar{at}wistar.upenn.edu.
This paper was submitted directly (Track II) to the PNAS office.
Data deposition: The sequence reported in this paper has been deposited in the GenBank database (accession no. AF221130).
Abbreviations:

WSTF,

Williams syndrome transcription factor;

EST,

expressed sequence tag